By Demetrios Papahadjopoulos, Paul R. Meers (auth.), Shinpei Ohki, Darrell Doyle, Thomas D. Flanagan, Sek Wen Hui, Eric Mayhew (eds.)
Membrane fusion is a crucial molecular occasion which performs a pivotal position in lots of dynamic mobile strategies, resembling exocytosis, endocytosis, membrane biogenesis, fertilization, and so forth. whereas many experiences at the physico-chem1cal method inquisitive about membrane fusion have seemed within the literature and masses details has amassed, there was no comprehensiv~ assembly of employees within the box. a up to date symposium which came about on the heart for day after today, nation college of latest York at Buffalo, long island, April 27-29, 1987, was once the 1st assembly of its style to in particular deal with the molecular mechanisms of membrane fusion. The Symposium consisted of oral, workshop and poster presentation periods. The papers awarded within the oral and workshop classes are accumulated right here and organized nearly within the order provided on the Symposium. those papers are the main updated and consultant paintings on the for entrance of every element of membrane fusion. even supposing the readers may possibly locate a few modifications in interpretations concerning the molecular mechanisms of membrane fusion, there's an over all concensus that elevated hydrophobicity and dehydration of the membrane floor are crucial physico-chemical components for membrane fusion to happen. We belief that those papers will give a contribution in your extra knowing of the mechanisms of membrane fusion.
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Additional info for Molecular Mechanisms of Membrane Fusion
St. Louis, MO) to form the monosodium salt of 1-(1,_13 C)-2-(1,_13 C)-dilauroyl-3-snphosphatidic acid was carried out using a procedure similar to one previously reported 12 . , St. Louis, MO). 3 mmol) were mixed in a small dry flask under nitrogen, melted (70 o C), and maintained as a stirred melt for 30 min. 2 mmo1) and dry chloroform (5 ml) were added. The flask was stoppered, sealed and protected from light and the reaction mixture stirred at room temperature until acylation was complete (1-3 hrs) as monitored by TLC.
The flask was stoppered, sealed and protected from light and the reaction mixture stirred at room temperature until acylation was complete (1-3 hrs) as monitored by TLC. 9% KCL in 1 N HCl (10 ml) and then washed several times with water/methanol (1:1). The crude product was collected from the chloroform layer and was isolated by fla~h chromotography using a solvent gradient (CHC13; CHC13/MeOH,l:1; CHC13/MeOH 1:2; CHC13/MeOH/H20, 65:35:8). Isolated yields were typically 80%. The purity was checked by analytical TLC developing solvent: chloroform/methanol/water, 65:35:8).
J. L. Browning and J. Seelig, Bilayers of Phosphatidylserine: A Deuterium and Phosphorus Nuclear Magnetic Resonance Study, Biochem. 19:1262 (1980). P. R. Cullis and B. De Kruijff, The Polymorphic Phase Behaviour of Phosphatidylethanolamines of Natural and Synthetic Origin. A 3l p NMR Study, Biochim. Biophys. Acta. 513:31 (1978). V. W. Miner and J. H. Prestegard, Structure of Divalent Cation-Phosphatidic Acid Complexes as Determined by 3l p _ NMR , Biochim. Biophys. Acta. 774:227 (1984). B. A. Cornell, Chemical shielding tensors of C13 in solid dimethyl oxalate, J.
Molecular Mechanisms of Membrane Fusion by Demetrios Papahadjopoulos, Paul R. Meers (auth.), Shinpei Ohki, Darrell Doyle, Thomas D. Flanagan, Sek Wen Hui, Eric Mayhew (eds.)