Comprehensive Virology: 17 Methods Used in the Study of by Max A. Lauffer (auth.), Heinz Fraenkel-Conrat, Robert R. PDF

By Max A. Lauffer (auth.), Heinz Fraenkel-Conrat, Robert R. Wagner (eds.)

ISBN-10: 1461566932

ISBN-13: 9781461566939

ISBN-10: 1461566959

ISBN-13: 9781461566953

The time turns out ripe for a serious compendium of that section of the organic universe we name viruses. Virology, as a technology, having handed just recently via its descriptive part of naming and num­ bering, has most likely reached that degree at which really few new­ actually new-viruses may be found. caused by means of the highbrow probes and strategies of molecular biology, genetics, biochemical cytology, and excessive solution microscopy and spectroscopy, the sphere has skilled a real details explosion. Few severe makes an attempt were made to chronicle those occasions. This entire sequence, on the way to include a few 6000 pages in a complete of approximately 18 volumes, represents a dedication by means of a wide crew of lively investigators to research, digest, and expostulate at the nice mass of knowledge on the subject of viruses, a lot of that's now amorphous and disjointed, and scattered all through a large literature. during this approach, we are hoping to put the total box in viewpoint, and to improve an invalua­ ble reference and sourcebook for researchers and scholars in any respect degrees. This sequence is designed as a continuum that may be entered at any place, yet which additionally presents a logical development of constructing proof and built-in concepts.

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It is almost exactly approximated by the ratio of the volume increase in a solution to the mass of solute added when a small mass is added to a large volume of solution. It is expressed in terms of mljg. When the volumes of solution and solute are strictly additive, the partial specific volume is equal to the specific volume, which in turn is equal to the reciprocal of the density of the added material. In the usual case, however, there are interactions between solute and solvent which cause volumes to be nonadditive.

The reaction shows that both strains combine with some of the same antibodies in the TMV type antiserum. Each strain also combines with antibodies that do not react with the other strain (Matthews, 1970). Reproduced with the permission of the copyright owner, Academic Press, Inc. Biophysical Methods in Virus Research 19 axis of rotation. This is called a centripetal force. Its magnitude is the mass of the object, m, times the acceleration toward the axis of rotation, w2x, where x is the distance along the string from the axis of rotation and w is the angular velocity in radians per second, which, in turn, is 211" times the number of revolutions per second.

It can be collected Biophysical Methods in Virus Research 41 in a fraction collector. As development continues, other bands will come off later and will be found in other fractions. Column chromatography can be used to purify virus preparations. LoGrippo (1951) showed that poliomyelitis and Theiler's viruses could be adsorbed onto and eluted from the basic resin, Amberlite Xe-67, and Shainoff and Lauffer (1956) used this same resin to remove the pigments from extracts of plants infected with southern bean mosaic virus.

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Comprehensive Virology: 17 Methods Used in the Study of Viruses by Max A. Lauffer (auth.), Heinz Fraenkel-Conrat, Robert R. Wagner (eds.)


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