By Hiroshi Maeda
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Additional info for Bismuth-based High-temperature Superconductors (Applied Physics Series , No 6)
This project focuses on four specific aims. First, the protein and lipid composition of the ER will be characterized in differentiating B cells and in a fibroblast model in which ER expansion is induced by enforced expression of XBP1(S). Second, the ability of the expanded ER to support protein biosynthesis will be evaluated in these systems. Third, the mechanism by which phospholipid biosynthesis increases during ER expansion will be investigated. Finally, factors that regulate ER biogenesis will be identified using biochemical and genetic approaches.
Such targeted inhibitors could significantly enhance the long-term prognosis of burn, cystic fibrosis, and HIV patients. To this end, the results here should enable novel therapies to treat and/or prevent P. aeruginosahuman infections. ; Professor and Chairman; Microbiology; University of Iowa Iowa City, Ia 52242 Timing: Fiscal Year 2006; Project Start 01-APR-2006; Project End 31-MAR-2007 Summary: (provided by applicant): This proposal requests funds for a JEOL JSM-7401F Field Emission Scanning Electron Microscope with embedded digital imaging, beam decelerating technology and high resolution backscattered electron imaging.
This project seeks to alleviate this bottleneck by creating E. coli strains that are more effective membrane protein producers. We plan to take a genetic approach, using a powerful selection for membrane protein expression that we have developed. By identifying and characterizing expression mutants we also plan learn more about the major barriers to expression. Our longer term goals are to apply the lessons learned here to other organisms, further broadening the range of membrane proteins that can be expressed at high levels.
Bismuth-based High-temperature Superconductors (Applied Physics Series , No 6) by Hiroshi Maeda