Read e-book online Artificial and Reconstituted Membrane Systems PDF

By Jacqueline A. Reynolds (auth.), J. R. Harris, A.-H. Etémadi (eds.)

ISBN-10: 1461393620

ISBN-13: 9781461393627

ISBN-10: 1461393647

ISBN-13: 9781461393641

This choice of eleven chapters is dedicated to a survey of man-made and reconsti­ tuted membrane platforms. those are basic topics and components of significant present significance in membrane biochemistry. in addition they relate good to the founding inspiration of this sequence, specifically, to offer experiences that gradually paintings towards and supply us with an "integrated view of the phone. " during this quantity, it's the software of a variety of physiochemical and biochemi­ cal ideas to the learn of membrane lipids and proteins which serves to illustrate the numerous development that has been made during this box during the last 25 years. From the certainty of simplified man made platforms, it really is was hoping that it'll finally be attainable to realize a extra exact figuring out of traditional organic membranes, in all their variety. This publication is a suitable successor to quantity thirteen of the sequence, which bargains with fluorescence stories on organic membranes. certainly, the current bankruptcy through Lesley Davenport and co-workers used to be initially due for inclusion in quantity thirteen, yet has been held over for inclusion during this quantity, the place it integrates remarkably good with the opposite subject matters. The tremendous assorted and fascinating contents of this quantity are actually in brief defined. In bankruptcy I, Jacqueline A. Reynolds and Darrell R. McCaslin pres­ ent a pertinent survey of the interplay of detergents with membrane lipids and proteins, including an evaluation of the reconstitution process.

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Biophysical Characterization of Membrane Lipid Polymorphism The polymorphic behavior of membrane polar lipids in aqueous systems has been extensively investigated using a variety of biophysical techniques. Each technique has its own particular advantages and drawbacks and, in general, the combination of several approaches to lipid phase determination is required to provide unambiguous phase assignments. In this section some applications of specific methods will be described and their usefulness in characterizing phase behavior of lipid-water systems will be discussed in the context of relevant examples.

7 x 10 cm, filled with an appropriate weight of the hydrophobic beads. The beads are washed exhaustively with buffer solution, and excess liquid is removed by applying gentle pressure with a syringe attached to a Luer fitting on the column reservoir cap. The sample containing mixed micell{;)s of detergent and lipid is then applied to the top of the column and rapidly forced through the moist beads by using the attached syringe. Removal of most of the aqueous solution from the beads prior to addition of the sample prevents undesirable dilution, and rapid passage of the sample minimizes lipid losses.

1970, Gel chromatography of proteins in denaturing solvents. J. BioI. Chem. 245:5166-5168. Green, D. , 1972, Membrane proteins: A perspective. Ann. Y. Acad. Sci. 195:150-172. Hartley, G. , 1936, Aqueous Solutions of Paraffin Chain Salts, Hermann et Cie, Paris. , 1975, Solubilization of membranes by detergents. Biochim. Biophys. Acta 415:29-79. , 1977, Reconstitution of Semliki Forest virus membrane. J. Cell Bioi. 75:866-880. , 1975, Three dimensional model of purple membrane obtained by electron microscopy.

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Artificial and Reconstituted Membrane Systems by Jacqueline A. Reynolds (auth.), J. R. Harris, A.-H. Etémadi (eds.)


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